The Novex® AP Chemiluminescent Substrate Enhancer consists of a 20X solution of Nitro-Block-II™ intended for use in conjunction with Novex® AP Chemiluminescent Substrate to increase the intensity of chemiluminescent signals when probing blots on nitrocellulose membranes. The enhancer produces a hydrophobic environment on the membrane which increases signal intensity and allows shorter exposure times for signal acquisition.