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NuPAGE® Novex 3-8% Tris-Acetate Midi-Gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex Tris-Acetate gels can also be run with Tris-Glycine Native Running Buffer to resolve native proteins more effectively than the Tris-Glycine gel system.
NuPAGE® Novex Tris-Acetate Midi-Gels (8 x 13 cm) can run up to 26 samples. The NuPAGE Midi-Gels run in the XCell4 Surelock™ Midi-Cell and, with a Midi Gel Adapter, can run in a Bio-Rad Criterion Cell.
Formulation: NuPAGE® Novex Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.
Recommended Buffers: Run NuPAGE® Novex Tris-Acetate gels with Novex® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Tris-Acetate gels.
12+2 wells Maximum sample volume/well 45 µl sample + 15 µl standards Maximum protein load Coomassie® Stain 0.7 µg/band Maximum protein load Silver Stain 1.4 ng/band
20 wells Maximum sample volume/well 25 µl Maximum protein load Coomassie® Stain 0.5 µg/band Maximum protein load Silver Stain 1 ng/band
26 wells Maximum sample volume/well 15 µl Maximum protein load Coomassie® Stain 0.35 µg/band Maximum protein load Silver Stain 0.7 ng/band
Currently using: Recommended NuPAGE® Gel: 4% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer) 6% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer) 10% Tris-Glycine NuPAGE® 8% Bis-Tris (+ MOPS Buffer) or NuPAGE® 10% Bis-Tris (+ MOPS Buffer) 12% Tris-Glycine NuPAGE® 10% Bis-Tris (+ MOPS Buffer) 4-12% Tris-Glycine NuPAGE® 3-8% Tris-Acetate (+ TA Buffer) or NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer) 4-20% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MES Buffer) 8-16% Tris-Glycine NuPAGE® 4-12% Bis-Tris (+ MOPS Buffer) 10% Tricine NuPAGE® 8% Bis-Tris (+ MES Buffer) or NuPAGE® 10% Bis-Tris (+ MES Buffer) 16% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer) 10-20% Tricine NuPAGE® 4-12% Bis-Tris (+ MES Buffer)
For more information on the benefits of NuPAGE neutral pH technology go to www.invitrogen.com/nupage
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