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Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00).
The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages • Stable expression • Long-term experiments • Accurate titer of functional virus • Flexible and versatile Gateway® recombination cloning technology
Key Features • HiPerform™ WPRE and cPPT elements • CMV promoter • V5 epitope tag at C terminus • Blasticidin selection
Kit includes • A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ • Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use.
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