Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells, Size A
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The Galacto-Star™ One-Step β-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.
• Glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used. • Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems. • Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range. • Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays. • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions. • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes. • Assay can be completed in about one hour, providing fast assay turnaround.
Sensitive The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Versatile The Galacto-Star™ system includes Galacton-Star® chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.
Long-Lasting Signal Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star® substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.
For Research Use Only. Not for use in diagnostics procedures.
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