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SYPRO® Ruby Protein Gel Stain is a highly sensitive, ready-to-use fluorescent stain for the detection of proteins separated by polyacrylamide gel electrophoresis (PAGE). SYPRO® Ruby stain is ideal for use in 1D and 2D PAGE. The sensitivity of SYPRO® Ruby Gel Stain is as good as or better than the best silver staining techniques, and this stain offers several advantages over silver staining:
• Simple staining procedure—no destaining or timed steps required • Linear quantitation range over three orders of magnitude • Compatibility with mass spectrometry and microsequencing
Stained proteins can be viewed with a standard UV or blue-light transilluminator or with a laser scanner. For optimal sensitivity with Polaroid film, use of the SYPRO® Photographic Filter (Cat. No. S-6656) is recommended.
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