Low Serum Growth Supplement (LSGS) is a sterile, concentrated (50X) solution intended for use as one component in a complete culture environment for the growth of human dermal fibroblasts and endothelial cells derived from large vessels. LSGS is intended for use with Medium 106 (fibroblasts) or Medium 200 (endothelial cells). LSGS contains: fetal bovine serum, basic fibroblast growth factor, heparin, hydrocortisone, and epidermal growth factor. When a 500 ml bottle of medium is supplemented with LSGS or the components of the LSGS Kit, the final concentrations of the components in the supplemented medium are: • fetal bovine serum, 2% v/v • hydrocortisone (1 µg/ml) • human epidermal growth factor, 10 ng/ml • basic fibroblast growth factor, 3 ng/ml • heparin, 10 µg/ml
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