A sterile, concentrated (100X) solution intended for use with EpiLife® medium or Medium 154 to culture human epidermal keratinocytes. HKGS is an ionically-balanced supplement containing bovine pituitary extract (BPE), bovine insulin, hydrocortisone, bovine transferrin, and human epidermal growth factor. Each 5 ml bottle of HKGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal keratinocytes, and is the correct amount of supplement for a 500 ml bottle of medium. When a 500 ml bottle of medium is supplemented with HKGS the final concentrations of the components in the supplemented medium are:
• bovine pituitary extract (BPE), 0.2% v/v
• bovine insulin, 5 µg/ml
• hydrocortisone, 0.18 µg/ml
• bovine transferrin, 5 µg/ml
• human epidermal growth factor, 0.2 ng/ml