NucRed™ Live 647 ReadyProbes™ Reagent is a cell-permeant nuclear stain that emits bright, far-red fluorescence when bound to DNA. Like other ReadyProbes™ reagents, NucRed™ Live 647 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

NucRed™ Live 647 ReadyProbes™ Reagent provides:

• Exceptionally bright far-red fluorescence well separated from blue, green, and red fluorophores
• Rapid staining of live or fixed cells without wash steps
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature— keep handy at your scope or cell culture area

Far-red Nuclear Stain for Cellular Imaging
The cell-permeable NucRed™ Live 647 reagent stains DNA and nuclei of live and fixed cells. It can be used for counter-staining, DNA content measurements, and cell counting. The far-red fluorescence makes it ideal for use in combination with blue, green, and red fluorophores, such as Hoechst, GFP/ FITC and Texas Red®.

Packaged for Convenience
NucRed™ Live 647 ReadyProbes™ Reagent is supplied ready-to-use and can be stored right next to your microscope or workstation. A liquid formulation in an ultra convenient dropper bottle (Figure 2) allows you to stain your cells whenever and wherever they are ready. Just add two drops per ml for optimally bright and distinct nuclear counter-staining.

Multiple Applications
The far-red spectral properties of NucRed™ Live 647 ReadyProbes™ Reagent (excitation /emission of 638/686 nm) make it ideal for use with blue (Hoechst, DAPI, CFP), green (Alexa Fluor® 488, FITC, GFP), and red (Alexa Fluor® 594, Texas Red®, rhodamine) fluorophores in multicolor experiments. Because of its high affinity for DNA, NucRed™ Live 647 ReadyProbes™ Reagent can also be used for cell counting, cell cycle, and cell replication studies.

Suggestions for Use

• NucRed™ Live 647 reagent may be added directly to cells in full media or buffer solutions
• No wash, cell lysis, or RNase steps are needed
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained. In most cases, staining intensity increases with time if cells are not washed prior to imaging.
• NucRed™ Live 647 dye is excited with a maximum at 638 nm when bound to DNA, with an emission maximum at 686 nm. It is detected through far-red filters, such as the Semrock 660/52 nm BrightLine® single-band bandpass filter.

See other Molecular Probes® imaging tools and reagents.