The Qubit® (previously known as Quant-iT™) Protein Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer (Q32866), but may be used with any fluorometer or fluorescence plate reader. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent. Common contaminants, such as reducing reagents (DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, or DNA, but not detergents, are well tolerated in the assay. Slight protocol modifications are required for other contaminants. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer.
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes
are required but not included.