The Qubit® (previously known as Quant-iT™) dsDNA HS (High Sensitivity) Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer (Q32866), but may be used with any fluorometer or fluorescence plate reader. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg⁄µl to 100 ng⁄µl. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes
are required but not included.