|CD45R (B220), rat IgG2a monoclonal|
|Qdot® 705 nanocrystal|
|Flow Cytometry, Immunoprecipitation|
Ex ⁄ Em and laser configuration:
|405 (488) ⁄ 705 nm, violet or blue laser|
Leukocyte Workshop Status:
Specificity for CD45R (B220)
The RA3-6B2 monoclonal antibody (mAb) reacts with an exon A-specific epitope on CD45 that is expressed on essentially all B cells and is maintained throughout B cell development. The RA3-6B2 epitope is expressed in a restricted manner by B cells; however, it is also present on lymphokine activated killer (LAK) cells. Although B220 has been widely used as a pan B cell marker, CD19 may be a more appropriate pan B cell marker, as expression of CD19 appears to be more closely restricted to B cells. The RA3-6B2 mAb has been shown to potentiate isotype switching in B cells and to inhibit proliferative responses of B cell mitogens on B cells. Applications of RA3-6B2 include immunostaining for flow cytometry and immunoprecipitation.
Use the primary antibody selector tool to see the full list of CD45R (B220) antibodies.
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