The CD4, clone S3.5, Qdot® 800 nanocrystal conjugate is a mouse monoclonal antibody (mAb) that recognizes the human CD4 antigen, also known as T4 and L3T4. The Qdot® 800 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and is most often used with 405 nm excitation, permitting simultaneous multicolor labeling of multiple targets off the violet laser. Alternatively, the nanocrystal can be excited by any laser up to the wavelength at which it absorbs.


Specificity for CD4
This antibody recognizes the CD4 antigen, also known as T4 and L3T4. CD4 is a cell surface receptor and a member of the immunoglobulin superfamily (IgSF). CD4 binds directly to MHC Class II molecules on the surface of antigen-presenting cells. As a co-receptor, CD4 assists the T-cell receptor (TCR) by recruiting tyrosine kinase Ick, an enzyme necessary to activate molecules involved in the signaling cascade of an activated T cell. CD4 is expressed on helper T cells, subsets of thymocytes, and to a lesser extent on peripheral blood monocytes. CD4 is also the known entry-point for HIV-1. This viral infection, which compromises the human immune system, leads to a progressive reduction of T-cells expressing CD4.

For Research Use Only
Not for use in diagnostic procedures.

Related Links
Use the primary antibody selector tool to see the full list of CD4 antibodies.
You can get additional information about Qdot® nanocrystals in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.