- Validated for use with Flow Cytometry applications.
- Qdot® nanocrystals are photostable and do not need to be protected from light.
- Qdot® nanocrystals exhibit intrinsic brightness that can often be greater than other fluorophores.
Each lot has been tested by flow cytometry using human peripheral blood leukocytes (PBLs). This testing was performed using 1 µL of antibody per 1 × 10e6 cells in a 100 µL staining volume (10 nM final concentration of Qdot® nanocrystals). Qdot® nanocrystal concentration is assigned based on optical density.
Get Brightness and Stability with Qdot® Nanocrystals
Qdot® nanocrystals are nanometer-scale atom clusters of semiconductor material which exhibit narrow and symmetrical emission bandwidths with very long Stokes shifts. The nanocrystals can be excited with any wavelength below their emission maximum, but are best excited by UV or violet (405 nm) light. Qdot® nanocrystals demonstrate an intrinsic brightness and photostability that can be many times greater than observed with other classes of fluorophores. These advantages make Qdot® nanocrystals powerful tools for antibody staining. The cytometer must have an appropriate emission filter for the Qdot® nanocrystal being used.
Easy Staining
Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA).
Specific for CD4
According to the literature, this antibody recognizes the CD4 antigen, also known as T4 and L3T4. CD4 serves as a co-receptor during T-cell activation through the binding of MHC Class II molecules, and is the receptor for HIV. CD4 is expressed on helper T cells, thymocyte subsets, and to a lesser extent on peripheral blood monocytes.
Leukocyte Workshop Status: Leukocyte Typing V
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
