The Qubit® (previously known as Quant-iT™) ssDNA Assay Kit is designed specifically for use with the Qubit® 2.0 Fluorometer (Q32866), but may be used with any fluorometer or fluorescence plate reader. The Qubit® ssDNA Kit is ideal for quantitating single-stranded DNA or oligonucleotides. However, it is not specific for single-stranded DNA. This assay kit will also detect double-stranded DNA and RNA but it will not detect contaminating protein or nucleotides. The assay is designed to be accurate for initial sample concentrations from 50 pg⁄µl to 200 ng⁄µl providing an assay range of 1–200 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes
are required but not included.