The Qubit® (previously known as Quant-iT™) RNA BR (Broad-Range) Assay Kit, when used with the Qubit® 2.0 Fluorometer (Q32866), provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations from 1 ng⁄µl to 1 µg⁄µl providing an assay range from 20–1,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the concentration using the Qubit® 2.0 Fluorometer.
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes
are required but not included.