The CD45R (B220), clone RA3-6B2, Qdot® 655 nanocrystal conjugate is a monoclonal rat antibody that recognizes the expressed product of the PTPRC gene. Qdot® 655 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser, or alternatively via excitation at 405 nm.


Specificity for CD45R (B220)
The RA3-6B2 monoclonal antibody (mAb) reacts with an exon A-specific epitope on CD45 that is expressed on essentially all B cells and is maintained throughout B cell development. The RA3-6B2 epitope is expressed in a restricted manner by B cells; however, it is also present on lymphokine activated killer (LAK) cells. Although B220 has been widely used as a pan B cell marker, CD19 may be a more appropriate pan B cell marker, as expression of CD19 appears to be more closely restricted to B cells. The RA3-6B2 mAb has been shown to potentiate isotype switching in B cells and to inhibit proliferative responses of B cell mitogens on B cells. Applications of RA3-6B2 include immunostaining for flow cytometry and immunoprecipitation.

Research Use Only
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Use the primary antibody selector tool to see the full list of CD45R (B220) antibodies.
You can get additional information about Qdot® nanocrystals in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.