The CD4, clone RM4-5, Qdot® 605 nanocrystal conjugate is a monoclonal rat antibody that recognizes the mouse CD4 antigen. The Qdot® 605 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser, or alternatively via excitation at 405 nm.


Specificity for CD4
The RM4-5 monoclonal antibody (mAb) reacts with CD4 antigen that is expressed on the majority of thymocytes and on the MHC class II restricted subset of mature T cells, including Th cells. Mouse CD4 antigen has also been reported to be present on multipotential hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic precursors. As a co-receptor in the TCR complex, CD4 is involved in T-cell activation through interaction with MHC class II on antigen presenting cells and in signal transduction via protein tyrosine kinase lck. The RM4-5 mAb can be used in immunostaining for flow cytometry and for immunohistochemistry on acetone-fixed, frozen tissue sections.

Research use only
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
Use the primary antibody selector tool to see the full list of CD4 antibodies.
You can get additional information about Qdot® nanocrystals in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.