The CD3, clone UCHT1, Qdot® 605 nanocrystal conjugate is a monoclonal antibody that is reactive to the human CD3 antigen. The Qdot® 605 nanocrystal conjugate may be used in the same way as conventional antibody conjugates and permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source—the 488 nm line of the argon-ion laser, or alternatively via excitation at 405 nm.

Antibody Specifications

Description:

CD3, Mouse IgG1 monoclonal

Label:

Qdot® 605 nanocrystal

Validated Application:

Flow Cytometry

Species Reactivity:

Human

Ex/Em and laser configuration:

405 (488)/605 nm, violet or blue laser

Clone ID:

UCHT1

Leukocyte Workshop Status:

Leukocyte Typing III, IV, and V

Regulatory Status:

RUO

Specificity for CD3
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR/CD3 complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by developing thymocytes and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 results in T-cell proliferation.

Research Use Only
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Related Links
For the full list of all CD3 antibodies available, please visit the primary antibody search tool.
You can get additional information about Qdot® nanocrystals in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.