New pH-sensitive pHrodo™ Red dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo™ Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
• Specific detection of phagocytosis and endocytosis • Reduced signal variability and improved timing in sensitive experiments • Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo™ Red Zymosan BioParticles® Conjugate in imaging or flow applications, or pHrodo™ Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
In addition, pHrodo™ dye is also available in green color, with E. coli, zymosan, S. aureus, and dextran 10,000 conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
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