New pH-sensitive pHrodo™ dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo™ dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
• Specific detection of phagocytosis and endocytosis • Reduced signal variability and improved timing in sensitive experiments. • Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo™ dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo™ E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo™ SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.