NuPAGE® Novex® 10% Bis-Tris Gels (1.0 mm thick, 10 well) arepre-cast polyacrylamide gels designed to give optimal separation ofyour small- to medium-sized proteins under denaturing conditions.Unlike traditional tris-glycine gels, NuPAGE® Novex® Bis-Trisgels have a neutral pH environment that minimizes proteinmodifications. Use the NuPAGE® Novex Bis-Tris Gels to prepareproteins for sequencing, mass spectrometry, and any other techniquewhere protein integrity is crucial. Also use NuPAGE® gels to getoptimal results for your day-to-day use. NuPAGE® Novex®Bis-Tris gels come in two sizes and multiple well formats. Each boxcontains 2 gels.

• Faster run times—separate your proteins in aslittle as 35 minutes
• Longer shelf life—NuPAGE® Novex® Bis-Tris gels canbe stored for at least 12 months at room temperature
• Better protein integrity—optimized sample preparationprocess preserves your proteins
• Wide range of molecular weight separation—select theright gel and running buffer to get the optimal separation of yourproteins

Choose the Right NuPAGE® Novex® Bis-Tris Gel for YourProtein Separation Needs
Get optimal separation of your proteins by choosing the rightcombination of gel and running buffer. NuPAGE® Novex® Bis-Trismini gels come in 3 polyacrylamide concentrations: 10%, 12%, and 4-12%gradient. We also offer 8% gels in the midi-gel format. The gels can berun using NuPAGE® MES or NuPAGE® MOPS SDS-running buffer toobtain different separation ranges. NuPAGE® Novex® Bis-Trisgels are based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamidegel, and have an operating pH of approximately 7.0.

Choose the Right NuPAGE® Novex® Bis-Tris Gel for Your Sample Size
Gels come in two sizes: mini (10 cm x 10 cm) and midi (8.7cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (minigel format only) in thickness. NuPAGE® Novex® Bis-Tris gelsalso come in multiple well formats.

Fast and Convenient to Use
NuPAGE® Novex® Bis-Tris Gels can be run in as little as 35 minuteswhen using the NuPAGE® MES running buffer. NuPAGE® Novex®Bis-Tris gels have a shelf life of at least 12 months at+4–25°C. Unlike traditional tris-glycine gels, NuPAGE®Novex® Bis-Tris gels have a neutral pH environment that minimizesprotein modifications. The gels provide sharper bands, higherresolution, longer shelf-life, and greater accuracy of results.

NuPAGE® Novex® Bis-Tris gels are formulated for denaturing gelelectrophoresis applications. For optimal sample preparation, use the NuPAGE®LDS Sample Buffer and NuPAGE®Sample Reducing Agent with your samples. Use NuPAGE®Antioxidant in the upper buffer chamber in order to maintain thereduced state of the proteins during the run and allow maximum bandsharpness. We recommend using Novex®Sharp Pre-stained Protein Standard; this protein standard containsour widest molecular weight range, with 12 proteins bands ranging from3.5–260 kDa. Get fast and sensitive staining of your protein gels withour SimplyBlue™SafeStain.

We also offerNuPAGE®Novex® Tris-Acetate gels for separating larger proteins. Forclassic Laemmli-based tris-glycine electrophoresis, we have Novex®Tris-Glycine gels.

NuPAGE® Novex® Bis-Tris gels can be used with the XCellSureLock™ Mini Cell and the XCell4 SureLock™ Midi Cell gelrunning apparatuses and are compatible with the iBlot geltransfer apparatus to create your western blots.

Related Links
• Overviewof 1D Protein Electrophoresis
• Comparisonof NuPAGE® Novex® Tris-Bis vs. traditional Tris-Glycine gels