NuPAGE® 4–12% Bis-Tris Gels (1.5-mm thick, 15-well) are pre-cast polyacrylamide gels designed to give optimal separation of your small- to medium-sized proteins under denaturing conditions. Unlike traditional tris-glycine gels, NuPAGE® Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use the NuPAGE® Bis-Tris Gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is crucial. Also use NuPAGE® gels to get optimal results for your day-to-day use. NuPAGE® Bis-Tris Gels come in two sizes and multiple well-formats. Each box contains 2 gels.
• Faster run times—get separation of your proteins in as little as 35 minutes
• Longer shelf life—NuPAGE® Bis-Tris gels can be stored for at least 12 months at room temperature.
• Better protein integrity—Optimized sample preparation process preserves your proteins.
• Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins.Choose the Right NuPAGE® Bis-Tris Gel for Your Protein Separation
Get optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE® Bis-Tris mini gels come in 3 polyacrylamide concentrations: 10%, 12%, and 4-12% gradient. We also offer 8% gels in the midi-gel format. The gels can be run using NuPAGE® MES or NuPAGE® MOPS SDS-running buffer to obtain different separation ranges. NuPAGE® Bis-Tris gels are based upon a Bis-Tris-HCl buffered (pH 6.4) polyacrylamide gel, and have operating pH of about 7.0. Choose the Right NuPAGE® Bis-Tris Gel for the Size of your Samples
Choose the right NuPAGE® Bis-Tris gels for the size of your sample. Gels come in two sizes: mini (10 cm x 10 cm) and midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE® Bis-Tris gels also come in multiple well-formats. Fast and Convenient to Use
NuPAGE® Bis-Tris Gels can be run as fast as 35 minutes when using the NuPAGE® MES running buffer. NuPAGE® Bis-Tris gels have a shelf life of at least 12-months at +4–25°C. Unlike traditional tris-glycine gels, NuPAGE® Bis-Tris gels have a neutral pH environment that minimizes protein modifications. The gels also provide sharper bands, high resolution, longer shelf-life, and higher accuracy of results.
NuPAGE® Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE® LDS Sample Buffer
and NuPAGE® Sample Reducing Agent
with your samples. Use NuPAGE® Antioxidant
in the upper buffer chamber in order to maintain the reduced state of the proteins during the run and allow maximum band sharpness. We recommend using Novex® Sharp Pre-stained Protein Standard
; this protein standard contains our widest molecular weight range with 12 proteins bands ranging from 3.5-260 kDa. Get fast and sensitive staining of your protein gels with our SimplyBlue™ SafeStain
We also provide the NuPAGE® Tris-Acetate gels
for separating larger proteins. For classic Laemmli-based tris-glycine electrophoresis, we provide Novex® Tris-Glycine gels
NuPAGE® Bis-Tris Gels can be used with the XCell SureLock™ Mini Cell and the XCell4 SureLock™ Midi Cell gel running apparatuses and are compatible with the iBlot gel transfer apparatus to create your western blots
• Overview of 1D Protein Electrophoresis
• Comparison of NuPAGE® Bis-Tris vs. traditional Tris-Glycine gels