The Newport Green™ DCF indicator has moderate zinc-binding affinity (Kd
~1 µM) but is essentially insensitive to Ca2+
(Kd for Ca2+
>100 µM), making this a valuable probe for detecting Zn2+
influx into neurons through voltage- or glutamate-gated channels. When used alongside dyes with dual Ca2+
sensitivity such as fura-2 and mag-fura-2, Newport Green™ DCF provides confirmation that changes in Zn2+
levels, and not Ca2+
, are being detected. The response of Newport Green™ DCF to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized.
Newport Green™ PDX has a higher Zn2+
dissociation constant (Kd
~30 µM) and a larger Zn2+
-free to Zn2+
-saturated fluorescence intensity increase than the Newport Green™ DCF indicators. Fluorescent Zinc Indicators Specifications:
• Label (Ex/Em): Newport Green™ PDX (~495/520 nm)
• Kd (Zn2+) (in buffer): ~30 µM
• Lyophilized product may be dissolved in DMSO for use
• Product is typically loaded into cells by adding the dissolved indicator to medium containing cellsFind Fluorescent Indicators for Zinc and Other Metal Ions
We offer a number of fluorescent indicators to determine polyvalent cation concentrations inside cells, to follow metal ion transport through ion channels, to make measurements in environmental samples, and more. Review Fluorescent Indicators for Zn2+ and Other Metal Ions—Section 19.7
in the Molecular Probes® Handbook for more information on these products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.