The Neon® Transfection System Starter Pack contains a Neon® Transfection device and two 192 reaction kits (10 µl and 100 µl sample sizes) to get you started in a fast, convenient, and cost effective manner. The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability.
The Neon® Transfection System is:
• Efficient—Up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
• Flexible—Easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
• Simple—Easy to use, with single reagent kit for all cell types
• Versatile—Open system allows electroporation parameters to be optimized freely
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. As shown in Figure 1, this design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (Kim JA, et al. 2008).
Plug-n-Play Out of the Box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.
Simplified Transfection Kit for All Cell Types
Avoid the hassle of determining which proprietary buffer kit will work with your favorite cell type. We have simplified your work with just one transfection kit that is compatible with all cell types. Start your transfection with our optimized protocols for popular cell types, or follow our standard simple optimization procedure for all new cell types.
Perform More Transfections with Limited or Precious Cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.
For Research Use Only. Not for any animal or human therapeutic or diagnostic use.