CD72.1 (Lyb-2.1), Mouse Anti-Mouse, (FITC); FITC conjugated Monoclonal antibody specific to Mouse CD72. This antibody is validated for use in Flow Cytometry. Anti-CD72 recognizes the expressed product of the CD72 gene.
Murine CD72.1⁄Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells.1,2 The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5⁄CD72 pairing.1 However, 10-1.D.2 can itself trigger a positive signal by binding CD72.1-5
Flow cytometry 1, 2
In vitro blocking and proliferation assays 2, 3
Luo, W., H Van de Velde, I. von Hoegen, J.R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630.
Subbarao, B., and D.E. Mosier. 1983. J. Immunol. 130:2033.
Snow, E.C., J.J. Mond, and B. Subbarao. 1986. J. Immunol. 137:1793.
Robinson, W.H., M.M Landolfi, H. Schaffer and J.R. Parnes. 1993. J. Immunol. 151:4764.
Beavil, A.J., R.L. Edmeades, H.J. Gould and B.J. Sutton. 1992. Proc. Nat. Acad. Sci. USA 89:753.