The CD8, clone 3B5, Pacific Orange™ dye conjugate is a monoclonal mouse antibody that recognizes the CD8 antigen also known as T8 and Lyt2,3. Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Orange™ and Pacific Blue™ dye conjugates can be simultaneously excited at 405 nm and emit at 551 nm and 455 nm, respectively, facilitating two-color analysis.

Antibody Specifications

Description:

CD8, Mouse IgG2a monoclonal

Label:

Pacitific Orange™ dye

Validated Application:

Flow Cytometry

Species Reactivity:

Human

Ex/Em and laser configuration:

405/551 nm, violet laser

Clone ID:

3B5

Leukocyte Workshop Status:

Leukocyte Typing V

Regulatory Status:

ASR

Specificity for CD8
This antibody recognizes the CD8 antigen also known as T8 and Lyt2,3. This antibody recognizes the α-chain alone as well as the αβ heterodimer. CD8 serves as a coreceptor during T-cell activation through the binding of MHC Class I molecules. CD8 is expressed on thymocyte subsets and cytotoxic T cells.

Analyte Specific Reagent
This product is an Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Links
Use the primary antibody selector tool to see the full list of CD8 antibodies.
You can get additional information about violet laser reagents in flow cytometry and use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.