The CD4, clone S3.5, Alexa Fluor® 488 conjugate is a monoclonal mouse antibody that recognizes the CD4 antigen also known as T4 and L3T4. Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute. The Alexa Fluor® 488 fluorescence emission profile is almost identical to that of fluorescein, and proivdes pH-insensitive fluorescence between pH 4 and 10.

Antibody Specifications

Description:

CD4, Mouse IgG2a monoclonal

Label:

Alexa Fluor® 488

Validated Application:

Flow Cytometry

Species Reactivity:

Human

Ex/Em and laser configuration:

495/519 nm, blue laser

Clone ID:

S3.5

Leukocyte Workshop Status:

Leukocyte Typing V

Regulatory Status:

ASR

Specificity for CD4
This antibody recognizes the CD4 antigen also known as T4 and L3T4. CD4 serves as a co-receptor during T-cell activation through the binding of MHC Class II molecules. CD4 is also the receptor for HIV. CD4 is expressed on helper T cells, thymocyte subsets, and to a lesser extent on peripheral blood monocytes.

Analyte Specific Reagent
This product is an Analyte Specific Reagent. Analytical and performance characteristics are not established.

Related Links
Use the primary antibody selector tool to see the full list of CD4 antibodies.
Use the Spectraviewer to help you plan your experiments. Additional general information about Life Technologies flow cytometry products and support is also available.