Host Species and Isotype:
Every Lot is Tested by Flow Cytometry
Every lot is tested by flow cytometry using freshly harvested mouse splenocytes. Because conditions may vary, it is recommended that each investigator determine the optimal amount of antibody to be used for each application.
Specific for CD8α (Ly-2)
The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α /β heterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive T cells. For this reason, antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive T cells. The 5H10 mAb has been used in other labs for flow cytometry and inhibition of T-cell responses to IL-2.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.