This singleplex bead kit is specifically designed for the measurement of human TNF-α in serum, plasma, and tissue culture supernatant. This assay may be run alone or in combination with other select Luminex® singleplex kits from Life Technologies. The panel is suitable for use with the Luminex® 100™/200™ and FLEXMAP 3D® systems. Buffer reagents needed to complete the reaction are sold separately (Catalogue number LHB0001) .
• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—fully qualified antibodies permit excellent specificity and sensitivity
• Fast and easy protocols—perform your assay and analyze your data typically in less than one day

Luminex® xMAP® Technology—Effective and Proven Analytical Tool
The TNF-α Human Singleplex Bead Kit is based on xMAP® technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex® assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex® 100™/200™ and FLEXMAP 3D® detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT® software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.

More Validation for Confidence in Your Results
The TNF-α Human Singleplex Bead Kit has been validated using the same rigorous criterion that distinguishes Luminex® assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below). Standard curves (Figures 1 and 2) are generated for each lot of product to assure quantitation consistency.

Learn more about the complete Luminex® product line.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.