The Human Th1/Th2/Th17 Magnetic 8-Plex Panel for the Luminex® platform is specifically designed for quantifying of human cytokines in serum, plasma, and tissue culture supernatant samples. By measuring 8 analytes simultaneously, the Luminex® assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay panel employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The panel is suitable for use with the Luminex® 200™, FLEXMAP® 3D, and MAGPIX® systems.

• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
• High quality—fully qualified antibodies permit excellent specificity and sensitivity
• Fast and easy protocols—perform your assays and analyze your data typically in less than one day

The Human Th1/Th2/Th17 Magnetic 8-Plex Panel for the Luminex® platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including: IL-2, IL-4, IL-5, IL-9, IL-10, IL-13, IL-17, and IFN-γ.

Luminex® xMAP® Technology—Effective and Proven Analytical Tool
The Human Th1/Th2/Th17 Magnetic 8-Plex Panel is based on xMAP® technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex® assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex® 200™ or FLEXMAP® 3D detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT® software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity. Alternatively, beads can also be read on the MAGPIX® system. This instrument is built on light-emitting diodes (LEDs) for excitation, a CCD camera for bead and analyte detection and high-speed digital signal processors. The MAGPIX® system is more compact providing the most cost effective magnetic bead-based multiplexing tool available.

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex® protocols, you can get your results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentrations, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead assays, enabling superior performance. A natural sample test comparing polystyrene and magnetic bead assays shows magnetic and polystyrene beads give equivalent results.

More Validation for Confidence in Your Results
The Human Th1/Th2/Th17 Magnetic 8-Plex Panel has been validated using the same rigorous criteria that distinguishes Luminex® assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay.

Learn more about the complete Luminex® product line