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SimplyBlue™ SafeStain is a ready-to-use, fast, sensitive, and safe Coomassie® G-250 stain for visualizing protein bands on polyacrylamide gels. This stain eliminates extensive solution preparation time and expenditure. Unlike traditional R-250 and some G-250 Coomassie® stains, SimplyBlue™ SafeStain is completely non-hazardous and does not require methanol or acetic acid fixatives or destains. Risk of hazardous exposure and unpleasant odors are eliminated, creating a healthier lab environment. The SimplyBlue™ SafeStain protocol is easy to perform and can be completed in less than 3 hours (Figure 1). For added speed, a simple microwave procedure can be completed in 12 minutes. Destaining is not required, but may be performed to achieve maximum sensitivity, especially when performing downstream analysis such as mass spectrometry or when a crystal-clear background is required. One microgram of protein can be detected after just 5 minutes in the stain. With additional water-based destaining, as little as 7 ng of protein (reduced BSA) can be detected (Figure 2).
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