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NuPAGE® Tris-Acetate SDS Running Buffer (20X) is formulated for separation of proteins, in their denatured state, on NuPAGE® Novex® Tris-Acetate gels. NuPAGE® Novex® Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE® Tris-Acetate SDS Running Buffer. NuPAGE® Novex® Tris-Acetate gels can also be run with Novex® Tris-Glycine Native Running Buffer to resolve native proteins more effectively than with the Tris-Glycine gel system.
Formulation NuPAGE® Novex® Tris-Acetate gels are made with high-purity, strictly quality-controlled reagents: Tris base, acetic acid, acrylamide, bis-acrylamide, TEMED, APS, and highly purified water. They do not contain SDS.
Recommended buffers Run NuPAGE® Novex® Tris-Acetate gels with NuPAGE® Tris-Acetate SDS Running Buffer. To ensure good sample reduction and band resolution, use NuPAGE® Sample Preparation Reagents with these gels. The use of NuPAGE® Transfer Buffer provides optimal conditions for transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis. For native running conditions, the Tris-Glycine Native Running and Sample Buffers should be used with NuPAGE® Novex® Tris-Acetate gels.
Easy-to-use format Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.
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