The LIVE/DEAD® Fixable Dead Cell Stain Kit employs an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow cytometry. In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity between the live and dead cell populations is typically greater than 50 fold, and this fluorescence intensity discrimination is completely preserved following formaldehyde fixation. These single-color assays use only one channel of a flow cytometer, making the reactive dyes in the LIVE/DEAD¤ Fixable Dead Cell Stain Kits compatible with multiparameter staining experiments. The violet-fluorescent reactive dye (L34955) has excitation and emission maxima of ~416/451 nm. Also available are LIVE/DEAD¤ Fixable Dead Cell Stain Kits that contain blue-fluorescent reactive dye (L23105), green-fluorescent reactive dye (L23101), and red-fluorescent reactive dye (L23102).
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.