- Easy to run
- Rapid protocol
- Convenient precoated, removable 8-well strips
The total assay incubation time is only 4 hours. A monoclonal capture antibody specific for Rat TNF-α has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Rat TNF-α content, controls, and unknowns, are pipetted into these wells. During the first incubation, the Rt TNF-α antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for Rt TNF-α is added. During the second incubation, this antibody binds to the immobilized Rt TNF-α captured during the first incubation. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Rat TNF-α present in the original specimen and the optical density can be read on a standard microplate reader.
Ready-to-Use Rat TNF-α ELISA Kit Contains These Reagents, Buffers, and Supplies:
- Rat TNF-α Antibody Coated 96-Well Plate
- Rat TNF-α Standard
- Standard Diluent Buffer
- Rat TNF-α Detection Antibody
- Streptavidin-HRP
- HRP Diluent
- Wash Buffer Concentrate (25X)
- Stabilized Chromogen, TMB
- Stop Solution
- Plate Covers
- Detailed protocol with validation tests
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want.
Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.
Part of the ELISA Kit Family
We have a wide menu of Life Technologies ELISA research use only kits. With excellent sensitivity, proven specificity, and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
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