TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include:
• 3´-T overhangs for direct ligation of Taq
-amplified PCR products
• Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro
RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
R I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
TOPO® TA Cloning® Kits are also available in combo kits
combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.