TOPO® TA Cloning® Kit, Dual Promoter, with One Shot® TOP10 Chemically Competent E.coli (No Manual)
Copy and paste this HTML to embed a link in your website
Paste link in email or IM
For fast, efficient cloning and subsequent in vitro transcription⁄translation, Invitrogen’s TOPO® TA Cloning® Dual Promoter Kit with our popular pCR®II vector is the only choice. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.
Convenient features of the TOPO® TA Cloning® Dual Promoter Kit with our pCR®II-TOPO® TA vector are:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products • T7 and SP6 promoters for efficient in vitro transcription • M13 forward and reverse primer sites for sequencing • 16 convenient and validated restriction sites including EcoR I flanking your insert for subsequent excision or sub-cloning • Kanamycin and ampicillin resistance for your choice of selection in E. coli • Easy blue⁄white colony screening for selection of recombinants • Includes OneShot® TOP10 F’ Competent cells for added convenience and highest cloning efficiency
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.