For fast, efficient cloning and subsequent in vitro transcription⁄translation, Invitrogen’s TOPO® TA Cloning® Dual Promoter Kit with our popular pCR®II vector is the only choice. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.

Convenient features of the TOPO® TA Cloning® Dual Promoter Kit with our pCR®II-TOPO® TA vector are:

• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient and validated restriction sites including EcoR I flanking your insert for subsequent excision or sub-cloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue⁄white colony screening for selection of recombinants
• Includes OneShot® TOP10 F’ Competent cells for added convenience and highest cloning efficiency