TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts. Some of the convenient features of the pCR® vectors are:

• 3´-T overhangs for direct ligation of Taq-amplified PCR products

• Choice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencing

• Versatile polylinker with flanking EcoR I sites for easy excision of inserts

• Choice of kanamycin or ampicillin selection

• Easy blue/white colony screening

• M13 forward and reverse primer sites for sequencing