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The Arcturus® HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for laser capture microdissection (LCM). Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.
Key product features: • Optimized process—provide superior staining while preserving RNA • Comprehensive kit—simplify tissue staining and dehydration • High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity • Versatile application—obtain high-quality RNA from many tissue types • Modular design—use with other Arcturus products to produce superior microarray data
Provide Superior Staining while Preserving Nucleic Acids HistoGene® Staining Solution has been developed by Arcturus® to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene® stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.
Simplify Tissue Staining and Dehydration The HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for LCM.
Retain Low-abundance mRNA RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene® LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene® samples appears free of degradation.
Maintain RNA Integrity Tissues prepared for LCM using HistoGene® LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.
Obtain High-quality RNA from Many Tissue Types Arcturus® has validated the HistoGene® Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene® Kit have yielded quality RNA. Validated tissue types include (Table 1):
Part of the Arcturus® System for Microgenomics The HistoGene® LCM Frozen Section Staining Kit is part of the Arcturus® Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure® RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. Arcturus® instruments and kits increase the reliability and reproducibility of gene expression studies.
For Research Use Only. Not for use in diagnostics procedures.
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