The Invitrogen VEGF Human ELISA Kit quantifies natural or recombinant human Vascular Endothelial Growth Factor (VEGF) in serum, plasma, buffered solution, or cell culture medium. VEGF is an important regulator of angiogenesis and vasculogenesis.

• Easy-to-run sandwich ELISA
• Results typically in one day--rapid 4-hour incubation protocol
• Pre-coated, removable 8-well strips
• Rigorous validation

The Invitrogen VEGF Human ELISA Kit includes all necessary reagents to run the assay:

• VEGF antibody-coated 96-well plates(s)
• Recombinant human VEGF standard and diluent
• Anti-VEGF Biotinylated detection antibody
• Streptavidin-HRP conjugate
• Stabilized chromagen
• Wash and incubation buffers
• Stop solution
• Plate covers
• Complete protocol

Get Accuracy with Simplicity
The Invitrogen Human VEGF kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A polyclonal antibody specific for human VEGF has been coated onto the wells of the
microtiter strips provided. Samples, standards, control specimens, and unknowns are pipetted into these wells. During the first incubation, the human VEGF antigen binds to the immobilized (capture) antibody. After washing, a biotinylated monoclonal antibody specific for human VEGF is added. During the second incubation, this antibody binds to the immobilized human VEGF captured during the first incubation. After washing, streptavidin-peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. Following a third incubation and washing, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of human VEGF present in the original specimen.

Get Results Quickly and Economically
The kit comes with the detection antibody pre-coated to the wells, eliminating the need for you to coat the plate. The incubation steps can be performed in 4 hours. No overnight incubation is needed. The pre-coated wells come as removable 8-well strips allowing you to tailor the assay size to your needs and reduce waste. Two standard vials per plate are provided to give you the option of reconstituting a new vial should you choose to not run the entire plate at one time.

Part of the Invitrogen ELISA Kit Family—Your Assurance of Quality
Invitrogen has a wide menu of ELISA kits targeted to disease-related proteins. Designed and validated for excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results. See product insert for specifics on validation. For research use only. Not intended for human or animal therapeutic or diagnostic use.

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