- Easy to run
- Convenient precoated, removable 8-well strips
- Validated kits for accuracy and consistency
The total assay incubation time is only 4 hours. A monoclonal capture antibody specific for Hu Tau has been coated onto the wells of the 96-well plate provided. During the first incubation, standards of known Tau content and unknown samples are pipetted into the wells and the Tau antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for Tau is added to the wells. During the second incubation, this antibody serves as a detection antibody by binding to the immobilized Tau protein captured during the first incubation. After washing, a horseradish peroxidase–labeled anti–rabbit IgG is added. This binds to the detection antibody to complete the four–member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu Tau present in the original specimen and the optical density can be read on a standard microplate reader.
Ready-to-Use Tau Total Human ELISA Kit Contains These Reagents, Buffers, and Supplies:
- Tau Antibody Coated 96-Well Plate
- Tau Total Standard
- Standard Diluent Buffer
- Tau Detection Antibody
- Anti-Rabbit IgG-HRP (100X)Â
- HRP Diluent
- Wash Buffer Concentrate (25X)
- Stabilized Chromogen, TMB
- Stop Solution
- Plate Covers
- Detailed protocol with validation tests
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to get quantitative results off the standard curve and/or can be used as a positive control.
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.
Part of the ELISA Kit Family
We have a wide menu of ELISA research use only kits.. With excellent sensitivity, proven specificity, and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.
For research use only. Not intended for human or animal therapeutic or diagnostic use.
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