The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO¤ Cloning. The pEF6/V5-His-TOPO® vector includes:

• Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells

• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines

• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody

• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)

• Antibody