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The pcDNA™3.3-TOPO® vector is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO® TA Cloning® Kit is supplied with the TOPO®-adapted plasmid vector, all reagents for cloning, and One Shot® TOP10 competent cells. The pcDNA™3.3-TOPO® vector offers the following benefits: • TOPO® adapted for cloning PCR products with >85% efficiency • High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp). • Vector is ideal for large scale protein expression. • Generation of native protein without extraneous amino acids
The pcDNA™3.3-TOPO® vector is also included with the OptiCHO™ Antibody Express System for cloning and expression of light and heavy antibody chains.
Storage: Store the pcDNA™3.3-TOPO® vector and all cloning reagents at -20°C. Store One Shot® TOP10 Chemically Competent E. coli cells at -80°C. When stored correctly, the kit is guaranteed for 6 months.
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