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MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3-10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:
• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time • Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2) • Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
Content and Storage:
MagicMedia™ E. coli Expression Medium is available in two convenient formats to meet specific needs-premixed powder packaged in a convenient, dissolvable bag (SoluPouch™ format) or a ready-to-use, presterilized liquid. Formats contain proprietary component A (in SoluPouch™ powder or 1X liquid format) and component B (in buffer format). Store the SoluPouch™ format at room temperature. Store the liquid format at +4°C. All components are guaranteed stable for 6 months when properly stored.
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