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The ViraPower™ HiPerform™ Lentiviral TOPO® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO® technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages • Stable expression • Long-term experiments • Accurate titer of functional virus • Typically provides 5 minute, high-efficiency TOPO® PCR cloning in three simple steps
Key Features • HiPerform™ WPRE and cPPT elements • CMV promoter • V5 epitope tag at C terminus •Blasticidin selection
Kit Includes • pLenti6.3⁄V5-TOPO® TA Cloning® Kit (K5315-20) • ViraPower™ Bsd Lentiviral Support Kit (K4970-00) • 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use.
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