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Invitrogen’s ViraPower™ Adenoviral Expression System facilitates highly efficient, in vitro or in vivo delivery of a target gene to dividing and non-dividing mammalian cells using a replication-incompetent adenovirus. ViraPower™ Adenoviral Expression System takes advantage of the Gateway® Recombination Cloning Technology to simplify cloning, which greatly enhances the efficiency of generating high-titer, recombinant adenovirus. This kit contains the pAd⁄CMV⁄V5-DEST Gateway®-adapted adenoviral vector enabling high level protein expression from human cytomegalovirus (CMV) immediate-early enhancer⁄promoter) as well as the 293A cell line.
Key advantages: • Produce high titer adenoviral stocks up to 1 x 1011 pfu⁄ml in concentrated preparations • High efficiency and rapid Gateway® Recombination Cloning bypasses inefficient homologous recombination in human or bacterial cells • Efficient delivery of the gene of interest to actively dividing and non-dividing mammalian cells in culture or in vivo • Amenable for high-throughput applications or library transfer applications • Produces of a replication-incompetent virus that enhances the biosafety of the system and its use as a gene delivery vehicle
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