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For fast, efficient subcloning in a variety of applications, the only choice is Invitrogen's TOPO® TA cloning Kit with our popular pCR®2.1 vector. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.
Convenient features of the TOPO® TA cloning Kit with our pCR2.1®-TOPO® TA vector are:3´-T overhangs for direct ligation of Taq-amplified PCR products • T7 promoter and M13 forward and reverse primer sites for in vitro RNA transcription and sequencing • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • 15 Convenient and validate restriction sites flanking your insert for easy, directional sub-cloning • Kanamycin and ampicillin resistance for your choice of selection in E. coli • Easy blue⁄white colony screening for selection of recombinants • Includes OneShot® TOP10 F' Competent cells for added convenience and highest cloning efficiency
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