TOPO® TA Cloning® Kit with pCR®2.1-TOPO®, One Shot TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit
Copy and paste this HTML to embed a link in your website
Paste link in email or IM
TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include: • 3´-T overhangs for direct ligation of Taq-amplified PCR products • Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing. • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli • Easy blue/white colony screening for selection of recombinants
TOPO® TA Cloning® Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.