pCR®8/GW/TOPO® TA Cloning Kit with One Shot® Mach1™-T1RE. coli
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The pCR®8/GW/TOPO® TA Cloning Kit delivers fast, efficient and reliable cloning. Additionally, this vector is ideal for use in Gateway® recombination cloning into various destination vectors for a multitude of applications.
This pCR®8 cloning kit offers the following features: • Fast, 5-minute TOPO® TA Cloning delivers 95% recombinants • 3´-T overhangs simplify cloning of Taq-amplified PCR products • Fast growing MachI cells shorten cloning time • Compatible with Gateway® recombination cloning enabling access to a multitude of destination vectors • Sequencing primer sites located close to cloning insertion site so you sequence more insert, less vector the first time • Spectinomycin resistance gene for robust selection in E. coli • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot® Mach1™-T1R Chemically Competent E. coli.
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