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The PureLink® Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns. • Fast – Ready-to-use plasmid DNA typically in about 30 minutes • Flexible – Formats work with vacuum and centrifugation platforms
Reliable and Cost-Effective Isolation of Plasmid DNA The PureLink® Quick Plasmid Miniprep Kit allows isolation of high quality plasmid DNA from 1-5 ml cultures in 30-45 minutes, using the PureLink® spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 µg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is then applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.
Compatible with Flexible Formats The PureLink® Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink® Quick Plasmid Miniprep Kit has an A260/A280 of < 1.80 when samples are diluted in Tris-HCl (pH 7.5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.
Isolated DNA can be used for a Number of Downstream Applications: DNA, purified using PureLink® Quick Plasmid Miniprep Kit is suitable for all routine downstream applications including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.
For Research Use Only. Not intended for diagnostic procedures.
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