GeneBLAzer® T-REx™ ROR gamma-UAS-bla HEK293T DA Kit
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The GeneBLAzer® T-REx™-ROR gamma-UAS-bla HEK293T DA cells contain tetracycline-inducible Gal4 DNA-binding domain⁄human ROR gamma ligand binding domain fusion and tetracycline repressor constructs stably integrated in the CellSensovr UAS-bla HEK293T cell line. CellSensor® UAS-bla HEK293T cells stably express a beta-lactamase reporter gene under the transcriptional control of a 7x Upstream Activator Sequence (UAS). Transcription from the 7xUAS is activated by the binding of the Gal4 transcription factor DNA-binding-domain (DBD). Addition of doxycycline, a tetracycline analog, allows for Gal4 DBD⁄ROR gamma LBD expression and subsequent beta-lactamase expression. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. This cell line has been functionally validated for Z’ and assay window, and have been tested for assay performance under variable conditions, including DMSO concentration, cell number, substrate loading time, and stimulation time.
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